RE: FW: H & E Staining problems
<< Previous Message | Next Message >>
From: | "Rich, Nina J Ms WACH" <Nina.Rich@se.amedd.army.mil> |
To: | "Rich, Nina J Ms WACH" <Nina.Rich@se.amedd.army.mil>, "'Weems, Joyce'" <JWEEMS@sjha.org>, "'Bill Sinai (Anatomical Pathology)'" <Bills@icpmr.wsahs.nsw.gov.au>, "'Colbert, Laurie'" <LColbert@phsca.org>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
-----Original Message-----
From: Rich, Nina J Ms WACH [mailto:Nina.Rich@se.amedd.army.mil]
Sent: Wednesday, March 29, 2000 9:12 AM
To: 'Weems, Joyce'; 'Colbert, Laurie'; 'Bill Sinai (Anatomical
Pathology)'; histonet@pathology.swmed.edu
Subject: RE: FW: H & E Staining problems
We had a similar problem a few years ago in a lab in Florida. It always
seemed to by the small biopsies that were affected. We discovered through a
QA Study with endoscopy that the specimens were beingplaced on gauze and
drying out before placing in formalin. We suggested that they use gauze
soaked in saline and then placing immediately into formalin. This solved
our problem and
completed the QA study. Hope this info is helpful to some of you.
Jean Rich
Winn Army Hospital
Ft. Stewart, Ga.
912-370-6083
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS@sjha.org]
Sent: Monday, March 27, 2000 10:50 AM
To: 'Colbert, Laurie'; 'Bill Sinai (Anatomical Pathology)';
histonet@pathology.swmed.edu
Subject: RE: FW: H & E Staining problems
I'm using Richard Allen Hematoxylin 7211 - Shandon's Instant Eosin - and
Stephen's Alcohols and formalin. (which come from Richard Allen through
Allegiance)
This will be interesting! Our problem started in 98 also, but we were using
different hematoxylin and eosin then - Surgipath Gills, and Surgipath eosin.
During trials, the docs liked the regressive stain so we continued with the
7211.
Anxious to hear if there's a trend. j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
-----Original Message-----
From: Colbert, Laurie [SMTP:LColbert@phsca.org]
Sent: Tuesday, March 21, 2000 3:35 PM
To: 'Bill Sinai (Anatomical Pathology)'; Colbert, Laurie;
histonet@pathology.swmed.edu
Subject: RE: FW: H & E Staining problems
We purchase all of our processing and staining products from Richard
Allan.
However, we have been having this problem since December '99 and
have gone
through many orders of formalin and other reagents.
Is Richard Allan a common denominator with everyone who is having
problems?????
Laurie Colbert
SJMC - Burbank, CA
-----Original Message-----
From: Bill Sinai (Anatomical Pathology)
[mailto:Bills@icpmr.wsahs.nsw.gov.au]
Sent: Monday, March 20, 2000 9:27 PM
To: Colbert, Laurie; histonet@pathology.swmed.edu
Subject: Re: FW: H & E Staining problems
Date: Mon, 20 Mar 2000 12:08:00 -0800
From: "Colbert, Laurie" <LColbert@phsca.org>
Subject: FW: H & E Staining problems
To: "HistoNet (E-mail)" <histonet@pathology.swmed.edu>
Dear Laurie,
This problem rears its ugly head occasionaly in Australia as well.
Nearly every time it can be followed back to a " BAD
BATCH of FORMALDEHYDE". Looking at Histonet lately I
feel this is happening to people in the USA. Are the people having
this problem all obtaining supplies from the one
supplier or initial source?
We fixed the problem by discarding our formalin
supplies, supplying a new batch and presto most of the
problems went away. We now only have the odd case which
causes problems (probably still some of the old batch). We supply
all our customers with formalin pots and concentrated solution from
our central site.
-----Original
Message-----From: Colbert, Laurie Sent: Friday, March 17, 2000 2:14
PM To: 'CMD1352@aol.com' Subject: RE: H & E Staining problems
We have had staining and/or processing problems off and on for
years, and we
are presently going through a very bad period where the problem is
not
correcting itself (which it always has in the past). Our sections
have very
faded nuclei mainly around the edges and an overall hazy look.
Several times
the pathologists have commented that the slides were too pink.
For every theory we come up with, we can always disprove that
theory. We do
histology for five different hospitals, and the blocks are processed
on
different processors. Our processing schedules are pretty much the
same on
each processor. But when we have problems, we have problems with
all of the
hospitals' blocks.
The main problem is with the small biopsies (skin, GI's, prostate
needle
biopsies, cervical biopsies). We think it may be an over-processing
problem, but when we got a demo processor so that we could set up a
short
run for the biopsies, we still had problems. The tissue looked
better, but
not great. One day the slides look as bad as they ever had. Our
routine
processing schedule is nine hours long. We have one formalin, two
PenFix,
three 100% alcohols, three xylenes, and three paraffins. We use no
heat
except on the paraffins.
We have approached the problem as a staining problem, but have
pretty much
ruled this out. When we sent our slides to other facilities for
staining,
they still looked bad.
We are at a total loss. Has anyone ever had water problems that
caused this
problem? We use tap water.
Laurie Colbert
Saint Joseph Medical Center
Burbank, Ca
(818) 557-5495
-----Original Message-----
From: CMD1352@aol.com [mailto:CMD1352@aol.com]
Sent: Thursday, March 16, 2000 7:27 PM
To: histonet@pathology.swmed.edu
Subject: H & E Staining problems
Our laboratory uses an automated Leica stainer for H & E. The
pathologist
has noticed several slides with weak staining around the periphery
of the
tissue section. The stain is very weak just along the edge of the
tissue.
The rest of the tissue stains fine. I checked the temperature of
the slide
dryer and found no problem. Any ideas how to solve this problem?
Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774 Fax 61+2+9687 2330
<< Previous Message | Next Message >>