Hi,
does anybody know who  makes DURA EDGE  microtome blades?

Renate
St. Mary's Hospital , Grand Junction, Co 81506

>----------
>From: 	HistoNet Server[SMTP:histonet@pathology.swmed.edu]
>Sent: 	Sunday, May 14, 2000 11:05 PM
>To: 	HistoNet Server
>Subject: 	Daily Digest
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:47:12 -0500
>From: Katri Tuomala <katri@istar.ca> (by way of Histonet)
>Subject: Test
>
>Test
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:47:31 -0500
>From: NLOUISEA@aol.com (by way of Histonet)
>Subject: testing
>
>testing
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:47:51 -0500
>From: "Eugene J. McCoy" <eugene.j.mccoy@worldnet.att.net> (by way of
>Histonet)
>Subject: Helicobacter Pyloric Stain
>
>Coni Forney  I Faxed you the procedure to do the best H. pyloric stain you
>will ever see.  At the bottom  of the page I put my phone number, if you
>need a little help with the procedure give me a call and I will help you
>enjoy the stain.  P.S. don't for get to make your Acidulated water first,
>its easy.
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:48:06 -0500
>From: ToniT41@aol.com (by way of Histonet)
>Subject: Halt in the water bath!!
>
>I have recently tried a solution called Halt.  It really helps get out the
>wrinkles....If any one wants to try it I got it from PolyScientific....They
>will send you a sample if you ask...
>I also haven't had any problems with any tissue washing off...I personally
>think the stuff is great...
>
>Toni
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:48:20 -0500
>From: ToniT41@aol.com (by way of Histonet)
>Subject: Alcian Yellow references
>
>Does anyone have any references for Alcian Yellow? They can be in Journals,
>or books.  Something that I can show my pathologist....geez...he drives me
>crazy!!!
>
>Thanks,
>Toni
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:48:43 -0500
>From: "ANN MARUSKA" <amarusk1@FAIRVIEW.ORG> (by way of Histonet)
>Subject: in situ
>
>Hi fellow Histonetters,
>
>Does anyone out there have experience with the instrument Vysis, used for
>FISH?  If you have any comments you would like to share, I would greatly
>appreciate it - and it will be kept completely confidential.
>TIA.
>
>Ann Maruska
>IHC Pathology
>Fairview-University Med Ctr.
>2414 South 7th Street
>Mpls. MN 55454
>612-672-4005
>amarusk1@fairview.org
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:48:58 -0500
>From: "Garza-Williams, Sara" <Garza-Williams.Sara@tchden.org> (by way of
>Histonet)
>Subject: Clothing etiquette for the bench tech.
>
>
>
>Please, please I need the your help regarding my argument to a CAP
>inspector (lab general).
>
>
>She gave me a huge "Ding" regarding the fact that our techs were not
>wearing lab coats(in the cutting and staining area), they do wear gloves. 
>She also justified her point by indicating because the techs were wearing
>jeans it was "unprofessional".  Of course, I could hardly contain myself
>and had try very hard to control myself from stating what I really wanted
>to say...
>
>
>I did tell her,  specimens were never triage in the main lab, so full PPE
>was irrelevant.  Our techs do wear nitrile gloves for protection from the
>reagents and chemicals. 
>
>
>She said full PPE was essential because the blocks and slides were
>considered infectious. So of course I said "At what point does the slide
>and/or block ever become non-infectious-when it reaches the pathologist?" 
>She said "They should continue to be considered infectious and that the
>pathologist should be wearing lab coats when they read out slides."  I said
>"Should the pathologist wear gloves as well, they have to touch the
>slide".  Our exchange went on and on until she said "I have to give you the
>deficiency".
>
>
>My pathologist went ballistic, as I did.  He and I would like to know what
>do other labs do?
>We are determined to argue for principle sake only.  My pathologist feels
>that PPE is essential when you consider every situation with logic but he
>doesn't care if the techs cut naked (metaphorically). in the lab because
>they do such good work and work safe.
>
>
>So my question is,  Do you require techs to wear a lab coat in the main
>part of the lab?  What is your lab policy?  Our policy (approved by the
>Infectious Disease department)  specifically states that no PPE is required
>in the main lab area, but full PPE is required in the gross room ,morgue or
>when cutting frozen sections.
>
>
>Of the hospitals that I've seen (a least a dozen) none of them require
>their techs to wear coats.
>I'd love the hear from other labs.  Sorry about the long story but I'm
>still steaming....
>
>
>Thanks
>Sara
>
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:49:13 -0500
>From: Connie McManus <conmac@cc.usu.edu> (by way of Histonet)
>Subject: Warthin Starry (was h. pylori stain)
>
>At 10:57 AM 05/11/2000 +0200, Buttigieg Carmen at MOH wrote:
>>Dear Dana
>>
>>Is it possible to have a copy of this technique + catalogue numbers of the
>>reagents. We currently use the Warthin Starry. Results are good but the
>>technique is long.
>
>
>I don't find the Warthin Starry to be a long stain to do.  What is your
>procedure?  Mine is very simple and I always get great results with it.
>
>
>
>Connie McManus
>Veterinary Diagnostics Lab
>Utah State University
>Logan, UT
>USA
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:49:33 -0500
>From: Jig1357@aol.com (by way of Histonet)
>Subject: Re: PAS DIASTASE
>
>We use diastase of malt, from Fisher.  I can get the catalogue number if you
>are interested.  .5 grams in 100 ml distilled water. Treat slides for 20 min.
> Another related question concerning diastase:
> I have worked several places and some will preheat the diastase solution to
>37 C then incubate the slides at that temp for 20-30 min.  Others just use
>the solution at room temp.   What are you folks doing for this procedure?
>Thanks!
>
>Jeanne Godine
>Pathology Consultants
>Lynchburg VA
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:49:49 -0500
>From: Amos Brooks <atbrooks@snet.net> (by way of Histonet)
>Subject: Re: Slide Adhesive for Bone
>
>Hi,
>    Don't use a microwave. At least an hour in a 60 degree oven should do the
>trick. Enzymatic predigestion for antigen retrieval is usually harsh. If
>you are
>using this try something different. 3% H2O2 is also harsh on many tissues try
>.03% for a longer time. If any tissue has survived your heating steps these
>suggestions may help keep it on the slides.
>Good Luck
>Amos Brooks
>
>Jay Turner wrote:
>
>> I've been having a lot of trouble with sections lifting during the heating
>> steps of anitgen retrieval for IHC stains on bone samples.  There isn't any
>> problem with the surrounding soft tissue (i.e. muscle), but the cortical
>> bone refuses to stay on the slide.  I've tried using Plus slides, coating
>> with poly-L-lysine, casein glue...almost everything that's out there.
>>
>> Does anyone have any suggestions for either an alternative slide adhesive
>>or
>> an alternative step that would be just as effective as HIER in bone?
>>
>> Thanks in advance.
>>
>> Jay
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:50:05 -0500
>From: Atoska Gentry <gentras@vetmed.auburn.edu> (by way of Histonet)
>Subject: ASH Spring Seminar
>
>	Rita Humphries or Larry Parks I need to talk to you about the ASH
>Spring
>Seminar  registration please contact me ASAP.  Thanks,  Atoska
>
>Atoska  S. Gentry B.S., HT(ASCP)
>Research Assistant II
>Scott-Ritchey RSCH Center
>Auburn University, AL  36849
>PH:   (334) 844-5579
>Fax:  (334) 844-5850
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:50:20 -0500
>From: "Horn, Hazel" <HornHazelV@exchange.ach.uams.edu> (by way of Histonet)
>Subject: RE: CPT code for Hpylori screens
>
>> We use CPT 87072 for the CLO Test   for h. pylori
>[Horn, Hazel]
>> -----Original Message-----
>> From:	MacDonald, Jennifer [SMTP:jmacdonald@sach.org]
>> Sent:	Thursday, May 04, 2000 03:57 PM
>> To:	'Histonet'; 'Goodwin, Diana'
>> Subject:	RE: CPT code for Hpylori screens
>>
>> Our pathologist charge an 88300 for a gross only.
>>
>> > ----------
>> > From: 	Goodwin, Diana[SMTP:DGoodwin@CHSNJ.org]
>> > Sent: 	Thursday, May 04, 2000 12:00 PM
>> > To: 	'Histonet'
>> > Subject: 	CPT code for Hpylori screens
>> >
>> > Hello, Histonetters.
>> >
>> > The weather here on the east coast, USA, has FINALLY gotten Spring-like
>> > and  we are enjoying a long-awaited drenching of sunshine and mild
>> > temperatures.
>> >
>> > Does anyone know if a CPT code exists for the reading and reporting of
>> > agar-type urease screen tests for H pylori, such as Clo-Test and hpFast,
>> > and also how to document these in workload tallies?
>> >
>> > Thanks in advance,
>> >
>> >
>> > Diana Goodwin,  HT
>> > Trenton,  NJ
>> >
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:50:39 -0500
>From: Anna Logvinova <alogvinova@buckcenter.org> (by way of Histonet)
>Subject: No mail today?
>
>Strangely I stopped recieving Histonet mail exchange today. Is there
>anything wrong with the server?
>Otherwise, please check my subscription. I miss it!
>
>Thanks,
>
>Anna
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:50:54 -0500
>From: RetTek2000@aol.com (by way of Histonet)
>Subject: TEST......2PM
>
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:51:11 -0500
>From: RetTek2000@aol.com (by way of Histonet)
>Subject: NO MESSAGES RECEIVED
>
>Are you down today. No messages are being received..........
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:51:26 -0500
>From: Greg Dobbin <dobbin@Upei.CA> (by way of Histonet)
>Subject: Hep. Growth Factor
>
>Hi All (again),
>
>I need to find an antibody to hepatocyte growth factor (HGF) that will
>work in FFPE tissues. Any suggestions?
>
>I will be staining canine livers. Anything to add? Any and all help
>greatly appreciated.
>
>Cheers! Greg
>
>>
>>
>>
>>
>>
>>
>Greg Dobbin
>Pathology Lab
>Atlantic Veterinary College, U.P.E.I.
>550 Unviversity Ave.
>Charlottetown, P.E.I.
>C1A 4P3
>Phone: (902)566-0744
>Fax: (902)566-0851
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:51:42 -0500
>From: Greg Dobbin <dobbin@Upei.CA> (by way of Histonet)
>Subject: Tyramide amplification
>
>Hi All,
>Could someone provide me with a brief explanation of the tyramide
>amplification technique and perhaps a reference or two, that would
>describe the method.
>
>I seem to recall there were patent problems/issues with copying the
>method. Has that all been sorted out? Is there a kit now comercially
>available?
>Cheers!  Greg
>>
>>
>>
>>
>>
>>
>Greg Dobbin
>Pathology Lab
>Atlantic Veterinary College, U.P.E.I.
>550 Unviversity Ave.
>Charlottetown, P.E.I.
>C1A 4P3
>Phone: (902)566-0744
>Fax: (902)566-0851
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:52:00 -0500
>From: stephen asquith <stephen.asquith@kcl.ac.uk> (by way of Histonet)
>Subject: anti-rat beta APP
>
>Dear all
>we have been using anti-rat beta APP from Boehringer on fixed frozen tissue
>with only limited results. Most of the work in the literature uses antigen
>retrival on paraffin sections.
>1.Is there a reason why the the antibody shouldn't work on the frozen tissue?
>2.Does anyone have another APP antibody that gives good cositant results.
>Any suggestions or comments greatly welcomed
>Thanks again.
>Steve
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:52:16 -0500
>From: taffy <hooser@anatomy.iupui.edu> (by way of Histonet)
>Subject: Platoid N
>
>Hello..AGAIN
>Does anyone have ANY idea as to a supplier for Plastoid N?  ANY LEADS ?
>Once more,
>THANKS
>Taffy Hooser
>
>
>
>
>
>Mary (Taffy) Hooser,  HT (ASCP)
>Indiana University School of Medicine
>Dept. of Anatomy and Cell Biology
>635 Barnhill Dr.  MS 5045 G
>Indianapolis, IN  46202
>
>Phone:  (317)274-7558
>Fax     : (317)278-2040
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:52:31 -0500
>From: Jill McVee <jm36@st-andrews.ac.uk> (by way of Histonet)
>Subject: Proliferating cell markers
>
>Hi histonetters,
>                 I have a Research Fellow who is in need of your global
>expertise. She would like to know if anyone has a proliferating cell marker
>anti-body
>which they use routinely  in  IC and is reliable. At the moment she is
>using anti-Brdu in mouse neuronal tissue  after in vivo injection but would
>like to change to a less invasive method of detecting proliferating cells.
>So if anybody out there has replaced their Brdu with another method please
>let me know.
>Jill McVee
>Histologist
>St.Andrews Uni
>Fife. Scotland.
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:53:02 -0500
>From: "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Histonet)
>Subject: Re: PAS DIASTASE etc
>
>On Thu, 11 May 2000 Eileen_Dusek@cdh.org wrote:
>
>> Does anyone have a procedure for Pas Diastase without using saliva.  In
>> this day and age of safety i am surprised we still use our own saliva.
>
>  In the absence of some active infection of the mouth, such as
>  streptococci, herpes simplex or Vincent's angina, it isn't easy
>  to think of saliva as unsafe. Even if you're a carrier of
>  meningococci or something potentially harmful to some others,
>  putting a drop of spit on a slide isn't exactly a promiscuous
>  and unregulated kiss-a-thon. For places that are short of cash,
>  such as hospitals in the middle of Africa and research laboratories
>  in Canada, saliva is a free, safe and very practical source of
>  the enzyme amylase (= diastase).
>
>  The arguments for using amylase from a chemical supplier are,
>  despite the truths in the preceding paragraph, quite strong.
>
>  (1) It isn't expensive (even in Canada) unless for some reason you
>      need some particular highly purified variant of the enzyme.
>      For confirming starch or glycogen, you don't.
>  (2) With bought amylase you can use a higher concentration than
>      your own salivary glands could ever put out, and consequently
>      the time to digest glycogen is shorter.
>  (3) Amylase isn't the only enzyme in spit. Proteolytic enzymes,
>      lysozyme and ribonuclease are other well known ingredients,
>      and there are surely many more. After all, this is the liquid
>      that cleaned people's teeth before the invention of the
>      toothbrush (except in India and perhaps elsewhere, where they
>      are lucky enough to have trees with suitably bristly twigs).
>
>  The other enzymes in saliva are unlikely to catalyze the hydrolysis
>  of substances in the section that would be stained by a method to
>  show glygogen (such as PAS or Best's carmine).
>
>  Instructions for making your own salivary RNase are in the 2nd
>  edition of Pearse's Histochemistry (1960). You heat to 90C for
>  a while, which inactivates all the other enzymes. I did this
>  once, years ago, and it worked: prevented the red staining of
>  neuronal RNA (Nissl substance) by methyl green-pyronine.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON,  Canada  N6A 5C1
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:53:39 -0500
>From: TWARE4U@aol.com (by way of Histonet)
>Subject: Re: PAS DIASTASE
>
>Dear Eileen,
>We use Poly Scientific's buffer and amylase sets. Unfortunately I am
>currently on maternity leave and I do not have the catalog numbers for you.
>If you have there catalog or if anyone out there can help I'd appreciate it.
>You don't have to use saliva it takes a little longer ,unless you have a
>microwave to speed things up a bit. I hope I helped you.
>Lisa
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:53:57 -0500
>From: Brown Alex <Alex.Brown@aaaht.scot.nhs.uk> (by way of Histonet)
>Subject: RE: h.pylori stain
>
>Hi Coni,
>	We use a modified Cresyl Fast Violet. We came across the method in a
>journal or paper a few years ago, unfortunately though the reference for it
>disappeared a long time ago.  ( If anyone recognises this technique I would
>appreciate the reference for it )
>Hope it's not subject to Copyright :#194#)
>
>
> Method :
>
> 1) Sections to water
> 2) Cresyl Violet solution			5 mins
> 3) Rinse in water
> 4) Rinse in 95% alcohol ( ethyl or methyl )
> 5) Differentiate in Cresyl Violet Differentiator until -
>	Nuclei		Violet
>	Cytoplasm	Almost colourless
>	Organisms	Deep Blue - Violet
> 6) Rinse in absolute alcohol
> 7) Clear and mount.
>
>Solutions :
>
>Cresyl Fast Violet - 	0.2% Cresyl Fast Violet Acetate in distilled water
>
>Differentiator          -		95% alcohol		90 ml
>			Chloroform		10 ml
>			Glacial Acetic Acid	  3 drops ( we use a
>disposable Pastette, so it's approx 0.15 ml )
>
>	Alex Brown
>	Crosshouse Hospital
>	Kilmarnock,Scotland.
>
> ----------
>From: Forney Constance M Civ 74 MDSS/SGSC
>To: 'HISTONET'
>Subject: h.pylori stain
>Date: Wednesday, May 10, 2000 6:12PM
>
>Looking for a quick and easy (ha ha) but excellent stain for h. pylori.
>
>Thanks,
>
>Coni Forney, BS MT,HT(ASCP)
>Technical Supervisor , Histopathology
>USAF Medical Center
>Wright Patterson, AFB, OH 45433
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:54:17 -0500
>From: terij@prlnet.com (Teri Johnson) (by way of Histonet)
>Subject: Re: PAS DIASTASE
>
>We use powdered Diastase and phosphate buffer from Rowley Biochemical.  It
>works quite well, especially in light of the fact we do so many of them.
>Just dissolve 0.1 g of Diastase in 100 ml of the buffer (pH 6.0).  Preheat
>to 37 degrees C, and place in the solution for one hour.  Rinse, and proceed
>with the PAS reaction.
>
>The catalog numbers are:  Diastase E-340-1, and Phosphate buffer, pH 6.0
>E-340-2.
>
>Good luck to you!
>
>- -Teri
>- ----- Original Message -----
>From: <Eileen_Dusek@cdh.org>
>To: <HistoNet@pathology.swmed.edu>
>Sent: Thursday, May 11, 2000 11:21 AM
>Subject: PAS DIASTASE
>
>
>> Hello everyone,
>> Does anyone have a procedure for Pas Diastase without using saliva.  In
>> this day and age of safety i am surprised we still use our own saliva.
>> I know Sigma has an Amylase Type IX-A, is anyone familar with using this
>> product, or any other type of diastase or amylase.
>> Thank you all very much.
>>
>> Eileen  Dusek
>> Central Dupage Hospital
>>
>>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:54:44 -0500
>From: Richard.Pitman@wri-tr.wmids.nhs.uk (by way of Histonet)
>Subject: Kevlar Autopsy  Gloves
>
>Hi All,
>
>Does anyone know a supplier of Kevlar gloves, suitable for use in the
>autopsy room ? Required to prevent needle stick injuries when sewing
>bodies. We have chain mail gloves already, but these are obviously no good
>for this particular task.
>
>Thanks,
>
>Richard
>
>Richard Pitman FIBMS,
>Head MLSO,
>Dept of Histology,
>Worcester Royal Infirmary
>Great Britain.
>
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:55:03 -0500
>From: "Buttigieg Carmen at MOH" <carmen.a.buttigieg@magnet.mt> (by way of
>Histonet)
>Subject: RE:Re: h.pylori stain
>
>Thanks to all for replying to my plea for an H. pylori stain. I think I'll
>try
>out the Tol blue/Alcian yellow for starters. It seems to be the most popular.
>
>Thanks again
>
>Carmen
>St. Luke's Hospital
>Malta
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:55:34 -0500
>From: Paul Klosen <klosen@neurochem.u-strasbg.fr> (by way of Histonet)
>Subject: Re: Protein extraction from paraffin blocks
>
>At 09:26 11/05/00 -0700, Victoria Baker a ecrit:
>>Hi All!
>>
>>The foundation is looking into extracting proteins
>>from paraffin/formalin fixed tissue samples.  Thus
>>far, the success is eh to not so hot.  I was wondering
>>if anyone had a procedure to do this, currently I
>>haven't found anything that really gave me an answer.
>>Given that the protein is masked by fixation, it seems
>>logical that their has to be a method to unmask it.
>>In IHC it's digestion, retrieval or both in a
>>combination.  If anyone has a source I could tap into,
>>I'd really appreciate it.
>
>If you use formalin-fixed tissue, it's quite normal to have some problems.
>The main problem is that -some of the proteins will be crosslinked, and
>will thus not migrate at the correct MW in electrophoresis. Also, the
>cross-linked proteins are difficult to extract. Better results can be
>obtained using non-additive fixatives like Carnoy or methacarn. I have done
>this a few years ago using 5% SDS to extract the dewaxed sections. In
>SDS-PAGE you still have a smear, but many proteins will give an almost
>correct appearance in Western Blots. If you want to have an idea on the
>results that can be obtained check Journal of Neurocytology 23: 297-311. We
>used this to check the specificity of anti-neurofilament monoclonals for
>different species. As we had some trouble obtaining fresh tissue for
>extraction, we used our paraffin embedded samples. This approach is also a
>much better control than using fresh tissue, as the proteins extracted from
>paraffin sections have gone to a complete histological processing, and are
>thus somewhat closer to the antigen present in the tissue sections.
>
>Paul
>                                                                         -=-
>                                                                        (o -)
>O
>===============================oOo==(_)==OOo=============================
>Paul Klosen, PhD
>CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
>Universite Louis Pasteur  12, rue de l'Universite
>F-67000 Strasbourg, FRANCE
>Tel. 03.88.35.85.04  Fax. 03.88.24.04.61
>========================klosen@neurochem.u-strasbg.fr========================
>=
>S
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:55:55 -0500
>From: "Buttigieg Carmen at MOH" <carmen.a.buttigieg@magnet.mt> (by way of
>Histonet)
>Subject: RE:PAS DIASTASE
>
>Dear Eileen
>
>We use 1% solution of Diastase from pig pancreas which we buy from BDH.
> The cat. no. is 39123 3N. We make it up fresh every day.
>It works perfectly for us.
>
>Good luck
>
>Carmen
>St. Luke's
>Malta
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:56:13 -0500
>From: "Nader, Alexander" <alexander.nader@wgkk.sozvers.at> (by way of
>Histonet)
>Subject: TIA-1 problems
>
>We have troubles with the TIA-1 Ab from Coulter. Although our dilution is
>1:1000, we still have sometimes positivity in reactive T-cells in
>bone-marrow biopsies.
>
>We use the DAKO-Kit as secondary Ab with DAB as chromogen.
>
>Dr. Alexander Nader
>Path. Institut Hanuschkrankenhaus
>A 1140 Wien, Oesterreich
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:56:33 -0500
>From: Brown Alex <Alex.Brown@aaaht.scot.nhs.uk> (by way of Histonet)
>Subject: RE: PAS DIASTASE
>
>Hi Eileen,
>	We use Sigma Amylase ( Type V1-B: from Porcine Pancreas ) We use it
>as a 1% solution in distilled water for 15mins at room temperature.
>	Alex Brown
>	Crosshouse Hospital
>	Kilmarnock, Scotland
>
> ----------
>From: Eileen_Dusek@cdh.org
>To: HistoNet@pathology.swmed.edu
>Subject: PAS DIASTASE
>Date: Thursday, May 11, 2000 5:21PM
>
>Hello everyone,
>Does anyone have a procedure for Pas Diastase without using saliva.  In
>this day and age of safety i am surprised we still use our own saliva.
>I know Sigma has an Amylase Type IX-A, is anyone familar with using this
>product, or any other type of diastase or amylase.
>Thank you all very much.
>
>Eileen  Dusek
>Central Dupage Hospital
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:56:54 -0500
>From: Nora Richards <niznr@unix.ccc.nottingham.ac.uk> (by way of Histonet)
>Subject: Automated IHS
>
>Thanks Sharon for reply - our autoantibody screening by indirect
>immunofluorescence is a bit different from histochemistry as we need to
>incubate patients serum with tissue sections as well, so the machines have
>to be adaptable for this - is your experience only histochemistry or have
>you ever seen this used for our application?
>
>Nora Richards, Immunology dept, Queens Medical Centre, Nottingham
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 13 May 2000 22:57:14 -0500
>From: "Louise Taylor" <louiset@mail.saimr.wits.ac.za> (by way of Histonet)
>Subject: RE: Coverglass thickness
>
>Hmmm,
>yes, there are plus factors regarding tape coverslippers BUT.... we have
>found that after a while the tape peels off, taking with it the section.
>This is especially serious for teaching and seminar cases  which are used
>year after year and represent rare and unusual material - so its glass still
>for all that.  I have also found that there a small irregularities in the
>tape which also make it difficult when looking at a section for fine details
>such as ISH signals. One has to keep focussing up and down to correct as one
>moves along.
>
>But generally I agree that there is substantial time saving involved.
>
>best regards
>Louise Taylor
>
>
>- -----Original Message-----
>From: a i d a n s c h u r r [mailto:Aidan.Schurr@hvh.co.nz]
>Sent: 11 May 2000 01:48
>To: Scott Taft; histonet@pathology.swmed.edu
>Subject: Re: Coverglass thickness
>
>
>>
>> PS- If you use tape, are you considering switching
>> back to glass?
>>
>> Scott Taft HT(ASCP)
>> Tucson, AZ
>>
>Scott - we use tape, and will *never* go back to glass!  The sheer
>convenience of the tape system hugely outweighs any loss of
>optical clarity (which is negligible if your microscope is adjusted
>correctly).  No more 'blocks' of slides stuck together in the file, no
>more endless hours fiddling with coverslips and DPX, no more
>racks of slides drying in ovens, no more cleaning DPX off 40x
>objectives when a pathologist goes too close to the edge of a wet
>slide!   oh joy, oh rapture!! (**big grin**)
>
>Aidan
>ps do you get the feeling I rather like this system!?
>___________________________________________________
>shin: device for finding furniture in the dark...
>___________________________________________________
>a i d a n   c   s c h u r r
>     mlso,  histology department
>      hutt valley health
>       lower hutt, new zealand
>
>     ph.  ++64 4 5709173
>     fax  ++64 4 5709214
>___________________________________________________
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 May 2000 06:27:17 -0500
>From: "Muhammad Tahseen" <tahseen@brain.net.pk>
>Subject: No message ?
>
>Hello,
>I am not getting any message from histonet since last two day?
>What happened to the list master
>Thanks
>Tahseen
>tahseen@brain.net.pk
>
>
>Here are the messages received yesterday!
>
>