We use CAM 5.2 from Becton Dickinson, cat. # 349205...(I'm sure they'd fax
you a product insert with all the technical info.)...We use Protease 2 for
16" on the instrument, 1 drop of antibody, 20" titration...and get very
strong staining.  For our controls we use breast and colon carcinomas, and
we also have some cytospins from a met. squam. cell carcinoma...I do have to
say, however, that the bottle is one of those that is extremely hard to
squeeze!  Let me know if there's anything else we can help you with on this
antibody.

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 
(608)265-6596
(608)263-1568(fax)

> -----Original Message-----
> From:	Sharon Bledsoe [SMTP:sbledsoe@iupui.edu]
> Sent:	Thursday, May 18, 2000 8:05 AM
> To:	Drew Sally A.
> Subject:	RE: CAM 5.2
> 
> We found the a reference to this antibody listed in a paper, but no 
> further infro.  We'd like to use this as a marker for kidney tissue.
> 
> I'd appreciate any information you could send me.
> 
> thanks,
> Sharon
> 
> 
> >What kind of info do you need?  If you'd like to know vendor, protocol,
> >control tissue, etc. that we use, just tell me and I'll list it all for
> you.
> >We use Ventana ES and genII...
> >
> >Sally Ann Drew-MT(ASCP)
> >Univ. of Wis. Hospital and Clinics
> >IHC/ISH Clinical/Research Lab
> >Madison, WI
> >(608)265-6596
> >(608)263-1568(fax)
> >
> >  > -----Original Message-----
> >  > From:	Sharon Bledsoe [SMTP:sbledsoe@iupui.edu]
> >  > Sent:	Wednesday, May 17, 2000 4:24 PM
> >  > To:	histonet@pathology.swmed.edu
> >  > Subject:	CAM 5.2
> >  >
> >  > Can anyone give me any information of CAM 5.2 antibody?
> >  >
> >  > Thanks