RE: Basic Comparison Questions
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From: | Brown Alex <Alex.Brown@aaaht.scot.nhs.uk> |
To: | HistoNet <Histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain |
Hi Marg,
We use the Leica Autostainer XL as follows :-
Oven 65degC 9 mins
Xylene 2 mins
Xylene 30 secs
Alcohol 30 secs
Alcohol 30 secs
Alcohol 30 secs
We could probably cut this down further, but it's quick enough for us.
The other stations are :
Haem 4.5 mins
0.1% Acid alcohol 10 secs
Lithium carbonate 20 secs
Eosin ( our own ) 1 min
Dehydration and clearing :
3 alcohols each 30 secs
Xylene 30 secs
Xylene 20 secs
Xylene Exit bath
As for the Haematoxylin, we use Shandon's Harris's ( acidified )
Hope this this helps,
Alex Brown
Crosshouse Hospital
Kilmarnock, Scotland.
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From: Hagerty, Marjorie A.
To: 'Histonet'
Subject: Basic Comparison Questions
Date: Monday, May 15, 2000 11:15PM
Hi,
I am interested in knowing the timing others use on the stations for the
Leica Stainer. I am particularly interested in the
deparaffinization/hydration and the oven. Trying to cut down some of the
times if possible.
Also, does anyone use a commercial Hematoxylin that they really like in any
kind of H&E set-up?
Thanks,
Marg
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