RE: Basic Comparison Questions

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From:Brown Alex <>
To:HistoNet <>

Hi Marg,
	We use the Leica Autostainer XL  as follows :-
Oven	65degC		9 mins
Xylene			2 mins
Xylene			30 secs
Alcohol			30 secs
Alcohol			30 secs
Alcohol			30 secs
We could probably cut this down further, but it's quick enough for us.
The other stations are :
Haem			4.5 mins
0.1% Acid alcohol		10 secs
Lithium carbonate		20 secs
Eosin ( our own )		1 min
Dehydration and clearing :
3 alcohols  each 	30 secs
Xylene		30 secs
Xylene		20 secs
Xylene		Exit bath

As for the Haematoxylin, we use Shandon's Harris's ( acidified )

	Hope this this helps,
		Alex Brown
		Crosshouse Hospital
		Kilmarnock, Scotland.

From: Hagerty, Marjorie A.
To: 'Histonet'
Subject: Basic Comparison Questions
Date: Monday, May 15, 2000 11:15PM


I am interested in knowing the timing others use on the stations for the
Leica Stainer. I am particularly interested in the
deparaffinization/hydration and the oven. Trying to cut down some of the
times if possible.

Also, does anyone use a commercial Hematoxylin that they really like in any
kind of H&E set-up?


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