Jennifer: I don't have any trouble doing alkaline phosphatase staining on
bone that was formalin fixed, formic acid decaled and paraffin embedded. I
use the method found in Bancroft & Cook (1994) Manual of Histological
Techniques and their Diagnostic Application, pg 300. It is a BCIP/NBT
method. I can fax you a copy if you like. Donna Montague, Orthopaedic
Research
University of Arkansas for Medical Sciences, Little Rock, AR

-----Original Message-----
From: Harvey, Jennifer [mailto:Jennifer_Harvey@URMC.Rochester.edu]
Sent: Monday, May 15, 2000 8:17 AM
To: 'Histonet'
Subject: Alk-Phos on mouse bones


Hi, 

I am looking for a procedure for Alkaline-Phosphatase on mouse bones. I am
under the impression it  will not work in paraffin. Am I correct? I can
process them in GMA or LR White. I am leaning to LR White. Has anyone every
stained Alk-Phos on LR White? 


Jennifer Harvey
University of Rochester
jennifer_harvey@urmc.rochester.edu