Mouse paws

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From:"Ryan.Linda" <ryan2@niehs.nih.gov> (by way of Histonet)
To:HistoNet@pathology.swmed.edu
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 Hi!
Anyone with experience decalcifying mouse limbs?  What type of decalcifier
do you use?  The researcher I'm helping is using a rapid decal solution
(RDO).  The paw is also not trimmed during the decal process and is embedded
whole(big mistake #1).  The sections look terrible.  I have a few
suggestions for her but would like more input from others on how you decal
and section rodent limbs.

Thanks

Linda Ryan, BS, HT(ASCP)HTL
National Institute of Environmental Health Sciences
111  T. W. Alexander Dr.
Bldg./rm.: 101/C-262
P.O. Box 12233
Research Triangle Park, NC 27709
(919) 541-4880(Laboratory)





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