IHC on bone

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From:Jane Chambers <jane.chambers@Agouron.COM>
To:HistoNet Server <histonet@pathology.swmed.edu>
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Dear Histonet,

I have experience doing IHC on paraffin embedded tissues but not on
bone.  We are going to want to do IHC on knee joints of mice.  Some of
the antibodies will work on paraffin embedded tissue; others on frozen
tissue.  We will contract this work out and I will get specifics from
whomever we go with.  However, I wanted to get some general information
just so I have an idea of what to plan for.  Do you just snap freeze the
bone in liquid nitrogen or place it some sort of media and then in a -80
freezer?  When cutting for frozen sections, do you have to decalcify the
bone first?  What type of microtome do you use?  Do most labs that do
IHC do it on frozen bone?

Thank you,

Jane Chambers

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