Gabba staining

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From:Colleen Forster <>
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Hello Histonet,

I have a couple of questions I am hoping that someone
out there can answer:

1. When doing perfusion on mouse with 80% alcohol,
how long should the removed tissue (brains) be left in
the fresh 80% after removal, for complete fixation?
When I perfuse I am runninmg the alcohol through the
body for 20 minutes /animal b/4 removing the brain.

2. In a much earlier post John Kiernan made reference
to using gluteraldehyde as a fixative for doing stains such
as GABBA. I am wondering what the best combination
for fixing tisuuse particularily for this group of AA is. Would
it be a stright % of Gluteraldehyde or a para/glut mix. I would
appreciate any help anyone might share on this antibody and the
best fixing and staining process.

As always, thank you in advance.

Colleen Forster
U of MN, Dept. of Neurology

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