GABBA and alcohol fix

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From:Colleen Forster <>
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          Gabba and alcohol fixation

Hello histoland,

I am reposting 2 questions that I have received no replies on.

1. Is anyone doing GABBA staining? If you are what type of
fixative are you using and if you are pretreating what method
are you using?

2. After profusing with 80% alcohol for 20 minutes I drop mouse
brains into fresh 80%. How long will they need to post fix in
alcohol before processing?

I hope the message goes through or I get at least one answer.

Thanks in advance.

Colleen Forster
U of MN

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