Bone Marrow Trephines

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From:Buttigieg Carmen at MOH <>
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Dear Histonetters

I need your help!

We routinely receive bone marrow trephines which are quite boney. We usually 
decalcify in EDTA but sometimes the endpoint is overshot and the cellular 
structures are ruined. We do not have a resin set-up, we process everything to 
paraffin. We do not have an x-ray set-up either.
Is there any way to cut a paraffin embedded bone marrow trephine on a manual 
rotary microtome without having to decalcify it?


St. Luke's Hospital

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