Bone Marrow Trephines
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From: | Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Dear Histonetters
I need your help!
We routinely receive bone marrow trephines which are quite boney. We usually
decalcify in EDTA but sometimes the endpoint is overshot and the cellular
structures are ruined. We do not have a resin set-up, we process everything to
paraffin. We do not have an x-ray set-up either.
Is there any way to cut a paraffin embedded bone marrow trephine on a manual
rotary microtome without having to decalcify it?
Thanks.
Carmen
St. Luke's Hospital
Malta
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