controls for rabbit polyclonals

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From:BonnieHylander@aol.com
To:Histonet@pathology.swmed.edu
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Plea for help,
     I am unable to get clean control slides for any rabbit polyclonal 
antibodies on either paraffin or frozen sections.  There is always background 
(often very dark) on the collagen, smooth muscle (especially prostate) etc.  
This happens with commercial antibodies (B2microglobulin or vonWillebrand), 
in house Abs, as well as with rabbit IgG.  I use casein or superblock. 
Avidin-Biotin block and exhaust endogenous peroxidase.  I use the ABC kit 
with DAB.  There is no problem if I omit the primary antibody.  Can anybody 
offer any insights?  Thank you, Bonnie



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