Russ,
  The hot water tends to speed up staining as chemical reactions speed up 
with the addition of heat.  This same practice can be applied during some 
H&E staining procedures...some bluing of the sections can be achieved if 
slides are rinsed in warm water after they leave Hematoxylin.

Carla Shoffeitt, B.S., H.T.(ASCP)
Histology Supervisor
Columbus Regional Medical Center
Columbus,GA.


>From: "RUSS ALLISON" <Allison@Cardiff.ac.uk>
>To: histonet@pathology.swmed.edu
>Subject: Re: special stains
>Date: Sun, 7 May 2000 11:04:18 GMT0BST
>
>I have never heard of the hot water wash after staining.  Please,
>somebody tell me about it.
>Also, why only after "special" stains?
>
>
>Russ Allison,
>Dental School
>Cardiff
>Wales
>

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