Linda,

DAKO have a monoclonal that I have used.  It works pretty well.  

Ann Thornton

At 11:56 AM 5/10/2000 -0500, you wrote:
>I've been working on a research project using a polyclonal Kip2 p57 antibody
>on prostate bxs.  I do get the expected nuclear staining but the
>non-specific staining is quite interfering.  My protocol does block for
>endogenous peroxidase and avidin-biotin.  The researcher has tried to find a
>monoclonal p57 to no avail but he doesn't have IPOX and Histonet at his
>disposal!  So I'm throwing this out to everyone out there as to whether
>they've used or heard of a monoclonal p57 that is applicalbe on FFPE tissue.
>
>Thanks in advance,
>
>Linda A. Sebree, HT
>University of Wisconsin Hospital & Clinics
>Immunohistochemistry/In Situ Hybridization Laboratory
>D4/218-2472
>600 Highland Avenue
>Madison, WI  53792-2472
>
>
>(608)265-6596
>FAX: (608)263-1568
>
>
>
>
>
Ann Thornton
Departmental Manager
Department of Pathology and Molecular Medicine
Wellington School of Medicine
Wellington 
New Zealand