Re: h.pylori stain

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From:Bryan Llewellyn <>
To:Histonet <>
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I have been using the following method for some years quite satisfactorily.
I originally got it off the University of Nottingham web site and adjusted
it to my liking.

Staining solution
1% aqueous toluidine blue  20-50 mL
pH 6.8 phosphate buffer 100mL

1. For use dilute 1 mL of the solution to 10 mL with distilled water (or
tap - I do).
2. Bring sections to tap water.
3. Stain in diluted stain for ten minutes or longer.
4. Wash thoroughly in tap water (couple of minutes).
5. Dehydrate, clear and mount.

Nuclei blue
Background pale blue
Organisms including helicobacter blue

The organisms are recognised by their shape.
Gram + and Gram - both stain blue
The pathologists in PG all find it OK.
Adjust the dye concentration and time to your preference.

Bryan Llewellyn

----- Original Message -----
From: Forney Constance M Civ 74 MDSS/SGSC <>
Sent: May 10, 2000 10:12 AM
Subject: h.pylori stain

> Looking for a quick and easy (ha ha) but excellent stain for h. pylori.
> Thanks,
> Coni Forney, BS MT,HT(ASCP)
> Technical Supervisor , Histopathology
> USAF Medical Center
> Wright Patterson, AFB, OH 45433

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