We do them the same. Toluidine blue in a borate solution, stain on a 
     hot plate for ~30 sec, then rinse with water, add a puddle of water 
     over the sections, and differentiate on the hot plate until the 
     plastic clears. Dry and mount. It works well for us.
                                        
     Jeffrey Crews, HTL (ASCP)
     Organogenesis, Inc.


______________________________ Reply Separator _________________________________
Subject: glycol methacrylate stain 
Author:  <KoellingR@immunex.com> at internet
Date:    05/04/2000 1:12 PM


Does anyone out there do toluidine blue routinely on their glycol 
methacrylate sections?  Can I employ the very same TB procedure on GMA as I 
use to stain my 1 micron epoxy resin sections.
     
Thank you.
     
Ray
Raymond Koelling
Immunex Corp.