Re: deplasticising

<< Previous Message | Next Message >>
From:Tammy Bird <VVTBird@ihc.com>
To:jlinda@ces.clemson.edu, histonet@pathology.swmed.edu
Reply-To:
Content-Type:text/plain; charset=US-ASCII

Anette have you thought of using Araldite resin to embed it.  It can be
removed and most stains done that could be done on paraffin.  

It has been several years since I have done anything with plastic so
much has changed.  We used Araldite for years on a routine basis, but it
was before many IHC studies were done so I don't know about them.  

We bought Araldite from Poly Scientific way back then.

Tammy

>>> Linda Jenkins <jlinda@ces.clemson.edu> 05/04/00 09:01AM >>>
Dear Anette,
	My first thought to your query of removing the PMMA and
replacing
with epoxy was,  "WHY on earth would you want to do that?"  In case
you
couldn't guess - I'm not a big fan of epoxy.  However, PMMA can easily
(but
slowly) be removed by returning, after trimming excess plastic, to the
liquid, inhibited methylmethacrylate monomer and stirring, stirring,
stirring(automatic stirrer suggested).  Remove "gummy" areas daily to
speed
up the process.  Please note that if you choose to embed in epoxy
that,
after a certain point of curing, you CANNOT remove the epoxy.  This is
based on a very bad personal experience with a one-of-a-kind
implant/tissue.
	Best Wishes,
	Linda
*********************************
Linda Jenkins, HT
Clemson University
Department of Bioengineering
864.656.5553
**********************************




<< Previous Message | Next Message >>