Re: dako peroxidase block
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From: | Paul Klosen <klosen@neurochem.u-strasbg.fr> |
To: | Gayle Callis <uvsgc@msu.oscs.montana.edu>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1"; format=flowed |
At 20:21 01/05/00 -0600, Gayle Callis a écrit:
>The Dako peroxidase block does contain hydrogen peroxidase, in fact, 0.03%,
>along with some sodium azide, approx. 0.01M, and is in buffer not methanol.
Looks a lot like the blocking solution published by Li et al. in JHC 35:
1457-1460 (0.1% sodium azide and 0.3% H2O2 in buffer). Works like a charm.
>Methanol is not advisable for CD markers on frozens, not only did it chew my
>sections off, but murine markers may not work, PBS with H2O2 is better.
>Biogenex and Pharmingen have both indicated this.
Many membrane antigens don't like being dehydrated. You take away the
membrane lipids necessary for the stabilization of the protein structure.
Just my 2 cents
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Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur 12, rue de l'Universite
F-67000 Strasbourg, FRANCE
Tel. 03.88.35.85.04 Fax. 03.88.24.04.61
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