Re: animal hair x-sections
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From: | "Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au> |
To: | Rhidian Harrington <r.harrington@pgrad.unimelb.edu.au>, "Histomail\\" <histomail@netspace.net.au>, histonet@pathology.swmed.edu |
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Content-Type: | text/plain; charset=US-ASCII |
Date: Mon, 08 May 2000 11:16:51 +1000
From: Rhidian Harrington <r.harrington@pgrad.unimelb.edu.au>
Subject: animal hair x-sections
To: histoNet@Pathology.swmed.edu
Many (!!!!!) years aog when I worked part-time for vet we rolled
bundles of hair in cigarette papers then processed the whole lot to
to paraffin. Cutting as many sections as you liked from the block.
We achieved about 80% of the hairs in cross section. I guess you
could use any of the current wet strength papers to perform the
same role.
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
I, with the help of Bruce Abaloz, have been trying to do
cross sections of animal hairs without much success. I collected the
hairs during a fauna survey in Pakistan. A cross section is the best
characteristic for identifying unknown animal hairs. The literature
(which is old) suggests that you use stainless steel slides with 0.8
mm holes drilled in them. The hairs are packed tightly in the holes
and then cut off on either side of the slide. A cover slip can be
mounted and the cross section viewed.
Does anybody know a different, more efficient method than this for
doing cross sections of hairs?
Thanks,
Rhidian
_________________________
Rhidian Harrington
Department of Zoology
University of Melbourne
Melbourne, Victoria
AUSTRALIA 3010
Ph: +61-3-8344 4354 (W)
+61-3-9844 2703 (H)
Fax: +61-3-8344 7909
Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774 Fax 61+2+9687 2330
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