"Coskran, Timothy M" wrote:

> Hello,
>
> Does anyone have any experience with using the V9 vimentin clone on mouse
> tissue?  We have had no luck getting any tissue that should be positive to
> label at all.  The mouse tissues are formalin fixed for 24 hours.  We have
> tried HIER, no antigen retrieval, and enzymatic methods. According to a
> cross-reactivity chart from DAKO, this clone should label mouse vimentin.

    A few years back a colleague of mine, Stan Baldwin, used V9 on frozen
sections, fixed in acetone. He said no other method of preparation worked. I
don't remember if he worked on mouse or rat. I think he is at Ohio State these
days. Try an e-mail to  sbaldwin@ee.net
    Good luck!

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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