Re: Tyramide amplification

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From:Heike Grabsch <>
To:"Greg Dobbin (by way of Histonet)" <dobbin@Upei.CA>
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Dear Greg, 

if you want to know about the tyramid amplification system (also 
called "CARD") please do a medline search with the name of Hopman-A. 
He has done a lot of work to establish a CARD system for 
immunhistochemistry as well as in situ hybridization. You will get a lot 
of very detailed articles and you will also get the recipe how to make 
your own tyramide amplification system. I met him in Basel during a 
workshop. he told me that DAKO and NEN did not like that he published 
how to make biotinylated tyramine/tyramid. But he said it is so easy 
(what is true, because I tried it) and so cheap compared to the kits 
availabe, that he did not care about patent problems. Of course, you 
could buy a kit to do it either from NEN called TSA-System or DAKO called 
Genpoint, but they are far too expensive. We do make our own with 
excellent results and very low costs. Please let me know if you are 
interested in more details. I coule send you the recipe and I could also 
send you some of Hopman's articles as attachment to my email if you are 
not able to copy them.

Dr. Heike Grabsch
Dept of Pathology
University of Duesseldorf

Greg Dobbin (by way of Histonet) wrote:
> Hi All,
> Could someone provide me with a brief explanation of the tyramide
> amplification technique and perhaps a reference or two, that would
> describe the method.
> I seem to recall there were patent problems/issues with copying the
> method. Has that all been sorted out? Is there a kit now comercially
> available?
> Cheers!  Greg
> >
> >
> >
> >
> >
> >
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 Unviversity Ave.
> Charlottetown, P.E.I.
> C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851

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