Re: Slide Adhesive for Bone

<< Previous Message | Next Message >>
From:Amos Brooks <> (by way of Histonet)
Content-Type:text/plain; charset="us-ascii"

    Don't use a microwave. At least an hour in a 60 degree oven should do the
trick. Enzymatic predigestion for antigen retrieval is usually harsh. If
you are
using this try something different. 3% H2O2 is also harsh on many tissues try
.03% for a longer time. If any tissue has survived your heating steps these
suggestions may help keep it on the slides.
Good Luck
Amos Brooks

Jay Turner wrote:

> I've been having a lot of trouble with sections lifting during the heating
> steps of anitgen retrieval for IHC stains on bone samples.  There isn't any
> problem with the surrounding soft tissue (i.e. muscle), but the cortical
> bone refuses to stay on the slide.  I've tried using Plus slides, coating
> with poly-L-lysine, casein glue...almost everything that's out there.
> Does anyone have any suggestions for either an alternative slide adhesive or
> an alternative step that would be just as effective as HIER in bone?
> Thanks in advance.
> Jay

<< Previous Message | Next Message >>