Re: Mayer's hematoxylin and chloral hydrate
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From: | Amos Brooks <atbrooks@snet.net> |
To: | Phyllis Davie <pdavie@phenopath.com> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi,
When you rinse your sections after counterstaining, use warm water. This
will give a very nice nuclear detail. :-)
Amos Brooks
Phyllis Davie wrote:
> I have been having trouble with the hematoxylin counterstain for my
> immunos. It is generally weak, with poor nuclear detail. I have been
> purchasing Mayer's hematoxylin, and I have tried 4 different vendors now,
> all with the same results. My first question is; what can be causing
> this weak staining, and what can I do to fix it? (I have been
> counterstaining from 4-15 minutes, and many times, the staining--poor as
> it is--drops off markedly after just a few racks of slides)
>
> The second part of my question is this; We decided to make our own
> Mayer's hematoxylin, but the recipe we found in the AFIP Laboratory
> Methods in Histotechnology, p. 52, calls for 50g of chloral hydrate.
> Unfortunately, chloral hydrate is a class IV DEA (Drug Enforcement
> Agency) regulated substance, and no-one here has a DEA license. Can
> anyone tell me what is the purpose of the chloral hydrate in the
> hematoxylin is?
>
> Thank you
>
> Phyllis Davie
> Clinical Laboratory Supervisor
> PhenoPath Laboratories
> Seattle, WA
> pdavie@phenopath.com
> (206)374-9009 phone
> (206)374-9009 fax
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