Re: Histofreeze
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From: | Amos Brooks <atbrooks@snet.net> |
To: | "Smith, Allen" <asmith@mail.barry.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi,
I avoid using the stuff at all costs. Sometimes it is necessary, but I try
to exhaust all other alternatives first.
freeze spray is for rookies!!!
Amos Brooks
"Smith, Allen" wrote:
> I concur with Andrea: Ice works better than Histofreeze. I usually protect
> my fingers with a paper towel and hold the ice cube against the block face
> in the (locked!) microtome.
> Allen A. Smith
> Barry University
> Miami Shores, FL
> -----Original Message-----
> From: Horn, Hazel [mailto:HornHazelV@exchange.ach.uams.edu]
> Sent: Tuesday, May 09, 2000 10:02 AM
> To: 'AndreaH@imclone.com'; HistoNet Server
> Subject: RE: Histofreeze
>
> Andrea's right..........iced water is the best way for sectioning for me!
> Hazel
>
> > -----Original Message-----
> > From: AndreaH@imclone.com [SMTP:AndreaH@imclone.com]
> > Sent: Monday, May 08, 2000 08:49 AM
> > To: HistoNet Server
> > Subject: RE: Histofreeze
> >
> >
> > I used to use a lot of Histofreeze spray and it became addictive to use
> > but
> > never really gave me better sections. Then I discovered putting the
> > blocks, after facing, on an iceblock that is melting a bit b/c it's at
> > room
> > temp and I have never used histofreeze sprays again ... better for me,
> > better for the block, and I get beautiful sections every time even with
> > finicky mouse tissues. My advice is to get rid of it. Plain ole ice works
> > much better.
> >
> > Andrea
> >
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