Dear Emma,
Contact your local cytology or microbiology labs. They regularly use 
a cytocentrifuge to spin down cells directly onto a slide. I would 
suggest you then do a PAP or H&E (donot dewax the slides - no wax 
there) on one slide fixed for 20min in 95% ethanol and a diff Quik or 
giemsa stain on an air dried slide.

Hope this helps,  Regards, Tony

> I was given "some cells" recently in eppendorf tubes and asked to stain and put them on slides to visualize under light microscope. As I have never worked with cells before, I had no idea where to st> Thanks in advance, Joyce Kotzuk, UNM pathology
> 
> >>> Emma Carter <E.Carter@OxfordBioMedica.co.uk> 04/26/00 09:40AM >>>
> 
> I am sure i read a message about this recently, but i cant recall, and i
> dont have anything in my mail folder...
> 
> someone has asked me about paraffin embedding some cells. I came up with a
> protocol off the top of my head, roughly:
> 
> Put cells in eppendorf tube, spin down and remove culture buffer.
> Resuspend with formalin and leave for 10 mins. Spin down and remove
> formalin.
> Resuspend/spin down through serial alcohols, then xylene.
> after final resuspension with xylene, remove and cover with warm wax.
> Allow wax pellet to set. Pop out of tube and embed in cassette.
> 
> How does this appear to other people? Should i go through the dehydration
> steps?
> 
> Any help would be very appreciated...... (oh yeah, this is all to be done by
> my own fair hand.......automation!? Pah!)
> 
> Thanks in advance,
> 
> emma carter
> 
> 
> 
> 
> 
> 
> 
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA

http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html