At 05.29 11/05/00 -0700, you wrote:
>
>I have to agree with Andi. coverglass thickness makes a big difference at
>the research level. We have to take photos that are publishable quality.
>Index of refraction etc. makes a big difference. Photos are not as forgiving
>as your eyes.  I believe everything in the light path should be taken into
>consideration including sample, coverglass, mountant and oil/air. The optics
>in most scopes (please someone correct me if I am mistaken) are set for 1.5
>standard coverglass especially for confocal microscopy. AJ
>
>

I would like to precise that manufactures agree that lenses are calculated
assuming a coverglass 0.17 mm thick.

Ciao Cristiano

_________________________________

Dr. Cristiano Rumio
Institute of Human Anatomy
Via Mangiagalli, 31
20133 Milan. 
Italy
Tel. -39-0270646243
Fax. -39-022364082
e.mail: cristiano.rumio@unimi.it

FLUORESCENCE 2000 MICROSCOPY COURSES
http://users.unimi.it/~fl2000/