Re: Coverglass thickness

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From:"Bill Sinai (Anatomical Pathology)" <>
Content-Type:text/plain; charset=US-ASCII

Date:          Wed, 10 May 2000 09:36:33 -0700 (PDT)
From:          Scott Taft <>
Subject:       Coverglass thickness
To:            HistoNet Server <>

Hello Histonetters,

I have another question of the day to pose:

Is there a reason, other than cost, why your lab would
prefer 1.0 thickness coverglass over 1.5 thickness? 
In the "old" days it was related to the optics of a microscope.  
Coverslip thickness above1.0 impeded the use of the higher 63x 
dry/oil or 100x oil.  I don't think there is al that much difference 
in cost.  With infinity corrected objectives this is less of a 
What is the reason your lab chooses what it currently

We have always used 1.0 coverslips.

How about your Pathologists'?
Our Pathologists rarely question scientific decisions we make!

PS- If you use tape, are you considering switching
back to glass?
Most definitely!!!
We are now using the 'new' tape but I have the feeling it will be no 
better than the old tape.  I have also trailed a tape from a Dutch 
company, but was less than satisfied with the result.  They 
apparently use a different resin which supposedly sets harder thus 
keeping the tape on longer??? 
Scott Taft HT(ASCP) 
Tucson, AZ

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Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital 
Phone 61+2+9845 7774  Fax 61+2+9687 2330

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