Re: Coverglass thickness
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From: | "Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au> |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Date: Wed, 10 May 2000 09:36:33 -0700 (PDT)
From: Scott Taft <ss336@yahoo.com>
Subject: Coverglass thickness
To: HistoNet Server <HistoNet@pathology.swmed.edu>
Hello Histonetters,
I have another question of the day to pose:
Is there a reason, other than cost, why your lab would
prefer 1.0 thickness coverglass over 1.5 thickness?
+++++++++++++++++++++++++++++++++++++++++++
In the "old" days it was related to the optics of a microscope.
Coverslip thickness above1.0 impeded the use of the higher 63x
dry/oil or 100x oil. I don't think there is al that much difference
in cost. With infinity corrected objectives this is less of a
problem.
++++++++++++++++++++++++++++++++++++++++++
What is the reason your lab chooses what it currently
uses?
++++++++++++++++++++++++++++++++++++++++++
We have always used 1.0 coverslips.
++++++++++++++++++++++++++++++++++++++++++
How about your Pathologists'?
++++++++++++++++++++++++++++++++++++++++++
Our Pathologists rarely question scientific decisions we make!
++++++++++++++++++++++++++++++++++++++++++
PS- If you use tape, are you considering switching
back to glass?
++++++++++++++++++++++++++++++++++++++++++
Most definitely!!!
We are now using the 'new' tape but I have the feeling it will be no
better than the old tape. I have also trailed a tape from a Dutch
company, but was less than satisfied with the result. They
apparently use a different resin which supposedly sets harder thus
keeping the tape on longer???
++++++++++++++++++++++++++++++++++++++++++
Scott Taft HT(ASCP)
Tucson, AZ
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Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774 Fax 61+2+9687 2330
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