Buttigieg Carmen at MOH wrote:
> 
> Dear Histonetters
> 
> Please help!
> 
> One of our pathologists just asked for a new procedure which I have never done
> before. He wants to stain up nerve fibres and cells containing
> acetylcholinesterase on frozen sections of intestine.
> I found a method in an old book where the incubation takes between 7 and 30
> days! Is there anything simpler?
> If anyone has the protcol could you please also send catalogue numbers of the
> not so common reagents which would certainly be involved.
> Thanks for your help.
> 
> Carmen
> St. Luke's Hospital
> Malta
> e-mail: carmen.a.buttigieg@magnet.mt
> Fax: 0356 224286

The most beautiful Acetylcholinesterase is the method by Karnovsky and
Roots!
Karnovsky, M.J. and Roots, L.: J. Histochem. Cytochem. 12, 219, 1964
If you like I will fax you my modified method.
Regards,
Markus F. Meyenhofer
Microscopy Labs
Box 338
Red Bank, NJ 07701
fax 732 758 9142