Patsy,

I block frozens for endogenous peroxidase by one of two methods that work
equally well with minimal damage to sections:

(1) 0.3% H2O2 in MeOH for 30 minutes at RT.
(2) DAKO's peroxidase block (not H202 based).

Andrea

-------------------------------------------------------------------

Date: 29 Apr 2000 13:14:51 -0500
From: Patsy.Ruegg@UCHSC.edu
Subject: 2 ihc questions

1.  does anybody do pap detection on frozen sections, if you do how do you
block for endogenous peroxidase, in my previous life we always avoided
hydrogen peroxide on frozen sections because it chewed up the tissue so
badly??????  enlighten me.

2.  does anyone have experience with doing ihc on alcohol fixed tissue
samples?  we have tons of archived bone samples fixed in cold 100% methyl
alcohol to preserve the fluorchrome label, that we would like to do some
IHC
on.  my past experience with this alcohol fixed tissue has been a dismal
failure.

thank you for your advice
patsy ruegg