Hi,
    Have you tried it without the epitope retrieval? Ours doesn't seem to need
it, as is the case with many muscle antigens.
Amos Brooks

Louise Taylor wrote:

> Hi histonetters (especially Zenobia downunder),
>
> I've got a problem with our MSA (muscle specfic antigen). It is producing a
> lot of background. The antibody is has been  frozen in aliquots , so I don't
> think that the problem can be contamination. I have followed the spec sheet
> recommendations of using Tris EDTA buffer, but all to no avail. I have
> always understood that freezing an antibody prolongs its shelf life
> (indefinately according to some) but could degradation account for the
> background? Any insights?
>
> Many thanks
> Louise taylor
>
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa