Michelle,
What are you embedding in paraffin? What is your fixation and processing
schedule?  How large are the embryos?
Pam Marcum

-----Original Message-----
From: Michelle Down [mailto:michellD@qimr.edu.au]
Sent: Friday, May 12, 2000 5:00 AM
To: 'HistoNet Server'
Subject: zebrafish


Hi all,
I was wondering what type of fixative people are using for zebrafish
embryos, especially for whole mount in situ hybridisation? I have been
using 4% paraformaldehyde in PBS and am getting a lot of wrinkly embryos.
Any suggestions would be much appreciated.
Thanks,
Michelle