RE: zebrafish

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From:Pam Marcum <>
To:Michelle Down <>, 'HistoNet Server' <>
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What are you embedding in paraffin? What is your fixation and processing
schedule?  How large are the embryos?
Pam Marcum

-----Original Message-----
From: Michelle Down []
Sent: Friday, May 12, 2000 5:00 AM
To: 'HistoNet Server'
Subject: zebrafish

Hi all,
I was wondering what type of fixative people are using for zebrafish
embryos, especially for whole mount in situ hybridisation? I have been
using 4% paraformaldehyde in PBS and am getting a lot of wrinkly embryos.
Any suggestions would be much appreciated.

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