RE: where are the CO2 microtomes

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From:"Ronnie Houston" <>
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Had one in back in Glasgow many moons ago, but haven't seen one since coming 
to the States.
We used it to cut frozen sections of brain for lipid histochemistry, and 
various metallic impregnation methods.
I loved it. Although the sections were thicker, in the 15-30 micron range, 
it was a real technical challenge to cut, stain and mount free-floating 
sections of whole cerebellum. Took a little time, but the rewards were more 
than ample. The younger techs don't know what they've missed by not having 
the opportunity to try one.
However, I imagine in the days where the money is so important, the time 
involved couldn't be justified; although I cannot imagine cutting cerebellum 
on a cryostat (fresh or fixed).
Just my opinion....sure someone will disagree.
Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital for Children
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