It has been awhile but I have had the to remove coverslips from HRP stained
slides and it fades only slightly.  I did make sure it was a limited
exposure to xylene and watched for the coverslip to come loose.  Pam Marcum

-----Original Message-----
From: a i d a n s c h u r r [mailto:Aidan.Schurr@hvh.co.nz]
Sent: Tuesday, May 02, 2000 6:51 PM
To: histonet@pathology.swmed.edu
Subject: re-DAB-ing slides


Hmmmm...

Does anyone know if the HRP survives the dehydrating process
when coverslipping?  ie. is it possible to de-coverslip an
'underdeveloped' slide, and re-incubate in DAB?  note: we have a
film coverslipper, so would be de-coverslipping in acetone - this
may be a negative factor!?

Thanks,
Aidan

___________________________________________________
a i d a n   c   s c h u r r
     mlso,  histology department
      hutt valley health
       lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214
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