RE: h.pylori stain
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From: | Brown Alex <Alex.Brown@aaaht.scot.nhs.uk> (by way of Histonet) |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
Hi Coni,
We use a modified Cresyl Fast Violet. We came across the method in a
journal or paper a few years ago, unfortunately though the reference for it
disappeared a long time ago. ( If anyone recognises this technique I would
appreciate the reference for it )
Hope it's not subject to Copyright :¬)
Method :
1) Sections to water
2) Cresyl Violet solution 5 mins
3) Rinse in water
4) Rinse in 95% alcohol ( ethyl or methyl )
5) Differentiate in Cresyl Violet Differentiator until -
Nuclei Violet
Cytoplasm Almost colourless
Organisms Deep Blue - Violet
6) Rinse in absolute alcohol
7) Clear and mount.
Solutions :
Cresyl Fast Violet - 0.2% Cresyl Fast Violet Acetate in distilled water
Differentiator - 95% alcohol 90 ml
Chloroform 10 ml
Glacial Acetic Acid 3 drops ( we use a
disposable Pastette, so it's approx 0.15 ml )
Alex Brown
Crosshouse Hospital
Kilmarnock,Scotland.
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From: Forney Constance M Civ 74 MDSS/SGSC
To: 'HISTONET'
Subject: h.pylori stain
Date: Wednesday, May 10, 2000 6:12PM
Looking for a quick and easy (ha ha) but excellent stain for h. pylori.
Thanks,
Coni Forney, BS MT,HT(ASCP)
Technical Supervisor , Histopathology
USAF Medical Center
Wright Patterson, AFB, OH 45433
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