RE: controls for rabbit polyclonals

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From:Abizar Lakdawalla <abizarl@innogenex.com>
To:BonnieHylander@aol.com, Histonet@pathology.swmed.edu
Reply-To:
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is this on human tissues?
abizar
www.innogenex.com
1-877-igx-info

-----Original Message-----
From: BonnieHylander@aol.com [mailto:BonnieHylander@aol.com]
Sent: Wednesday, May 03, 2000 3:22 PM
To: Histonet@pathology.swmed.edu
Subject: controls for rabbit polyclonals


Plea for help,
     I am unable to get clean control slides for any rabbit polyclonal
antibodies on either paraffin or frozen sections.  There is always
background
(often very dark) on the collagen, smooth muscle (especially prostate) etc.
This happens with commercial antibodies (B2microglobulin or vonWillebrand),
in house Abs, as well as with rabbit IgG.  I use casein or superblock.
Avidin-Biotin block and exhaust endogenous peroxidase.  I use the ABC kit
with DAB.  There is no problem if I omit the primary antibody.  Can anybody
offer any insights?  Thank you, Bonnie





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