RE: controls for rabbit polyclonals

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From:Abizar Lakdawalla <>,
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is this on human tissues?

-----Original Message-----
From: []
Sent: Wednesday, May 03, 2000 3:22 PM
Subject: controls for rabbit polyclonals

Plea for help,
     I am unable to get clean control slides for any rabbit polyclonal
antibodies on either paraffin or frozen sections.  There is always
(often very dark) on the collagen, smooth muscle (especially prostate) etc.
This happens with commercial antibodies (B2microglobulin or vonWillebrand),
in house Abs, as well as with rabbit IgG.  I use casein or superblock.
Avidin-Biotin block and exhaust endogenous peroxidase.  I use the ABC kit
with DAB.  There is no problem if I omit the primary antibody.  Can anybody
offer any insights?  Thank you, Bonnie

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