RE: To steam or to pressure cook??

<< Previous Message | Next Message >>
From:Cynthia Favara <cfavara@niaid.nih.gov>
To:"'Marylou Pohl'" <mbpohl@acsu.buffalo.edu>, Histonet@pathology.swmed.edu
Reply-To:
Content-Type:text/plain; charset="iso-8859-1"

Marylou,
    I happen to have steamer microwave and autoclave in my lab. Started with
a generic microwave no turn table and found that even with a standard volume
temp and number of slides we had variation in some of our staining. Steamer
was next; easier to control temp and vary number of slides but still had
some variation in staining. Have progressed to a microwave by Energy Beam
Scientific with probe and bubbler, completely submerge the slides set the
temp to 95C [just below boiling at this altitude] and timer for 30minutes.
Staining has been more consistent and has made my life easier. I also use
hydrolytic autoclaving and autoclaving with distilled water for some of my
antibodies. My preference is a microwave that can have the temp and time
set---much easier on my gray hairs. 
Cynthia Favara 
NIAID/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
PH: 406-363-9317 
FAX: 406-363-9286 

e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov> 

 




<< Previous Message | Next Message >>