RE: To steam or to pressure cook??
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From: | Cynthia Favara <cfavara@niaid.nih.gov> |
To: | "'Marylou Pohl'" <mbpohl@acsu.buffalo.edu>, Histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
Marylou,
I happen to have steamer microwave and autoclave in my lab. Started with
a generic microwave no turn table and found that even with a standard volume
temp and number of slides we had variation in some of our staining. Steamer
was next; easier to control temp and vary number of slides but still had
some variation in staining. Have progressed to a microwave by Energy Beam
Scientific with probe and bubbler, completely submerge the slides set the
temp to 95C [just below boiling at this altitude] and timer for 30minutes.
Staining has been more consistent and has made my life easier. I also use
hydrolytic autoclaving and autoclaving with distilled water for some of my
antibodies. My preference is a microwave that can have the temp and time
set---much easier on my gray hairs.
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286
e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov>
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