RE: Re: Acetylcholinesterase
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From: | "Johnson, Mickey" <JohnsoM@shmc.org> |
To: | "'Horn, Hazel'" <HornHazelV@exchange.ach.uams.edu>, "'Paul Klosen'" <klosen@neurochem.u-strasbg.fr>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
Hi Hazel,
Could you also FAX me this procedure too?
Thank you.
Mickie
Michael L Johnson, BS, HTL(ASCP)
Histology Supervisor
Department of Pathology
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, WA 99220
johnsom@shmc.org
FAX (509)474-2052
-----Original Message-----
From: Horn, Hazel [mailto:HornHazelV@exchange.ach.uams.edu]
Sent: Thursday, May 04, 2000 7:33 AM
To: 'Paul Klosen'; histonet@pathology.swmed.edu
Subject: RE: Re: Acetylcholinesterase
I have a very good procedure for this............send me your fax number and
I will fax it to you.
Hazel Horn
> -----Original Message-----
> From: Paul Klosen [SMTP:klosen@neurochem.u-strasbg.fr]
> Sent: Thursday, May 04, 2000 06:16 AM
> To: histonet@pathology.swmed.edu
> Subject: Fwd: Re: Acetylcholinesterase
>
>
> >Date: Thu, 04 May 2000 13:09:17 +0200
> >To: Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt>
> >From: Paul Klosen <klosen@neurochem.u-strasbg.fr>
> >Subject: Re: Acetylcholinesterase
> >
> >-At 09:57 04/05/00 +0200, vous avez écrit:
> >>Dear Histonetters
> >>
> >>Please help!
> >>
> >>One of our pathologists just asked for a new procedure which I have
> never
> >>done
> >>before. He wants to stain up nerve fibres and cells containing
> >>acetylcholinesterase on frozen sections of intestine.
> >>I found a method in an old book where the incubation takes between 7 and
> 30
> >>days! Is there anything simpler?
> >
> >Hi
> >
> >It's been a few years since I've done AChE histochemistry, but there are
> >certainly many protocols a lot shorter than the one you mentionned.
> >Personnally, I prefered the protocol published by Di Patre et al. in JHC
> >41 (1993): 129-135. Very sensitive, easy and most reagents are cheap. I
> >have a protocol, but unfortunately it's in French. Otherwise I can fax
> you
> >the material and methods section of the reprint.
> >
> >Paul
>
>
> -=-
> (o
> -) O
> =3D========================3D======oOo==(_)==OOo===========3D==================
> Paul Klosen, PhD
> CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
> Universite Louis Pasteur 12, rue de l'Universite
> F-67000 Strasbourg, FRANCE
> Tel. 03.88.35.85.04 Fax. 03.88.24.04.61
> =3D=======================klosen@neurochem.u-strasbg.fr================3D=====
> ====
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