RE: Re: Acetylcholinesterase

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From:"Horn, Hazel" <HornHazelV@exchange.ach.uams.edu>
To:'Paul Klosen' <klosen@neurochem.u-strasbg.fr>, histonet@pathology.swmed.edu
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I have a very good procedure for this............send me your fax number and
I will fax it to you.
Hazel Horn

> -----Original Message-----
> From:	Paul Klosen [SMTP:klosen@neurochem.u-strasbg.fr]
> Sent:	Thursday, May 04, 2000 06:16 AM
> To:	histonet@pathology.swmed.edu
> Subject:	Fwd: Re: Acetylcholinesterase
> 
> 
> >Date: Thu, 04 May 2000 13:09:17 +0200
> >To: Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt>
> >From: Paul Klosen <klosen@neurochem.u-strasbg.fr>
> >Subject: Re: Acetylcholinesterase
> >
> >-At 09:57 04/05/00 +0200, vous avez écrit:
> >>Dear Histonetters
> >>
> >>Please help!
> >>
> >>One of our pathologists just asked for a new procedure which I have
> never 
> >>done
> >>before. He wants to stain up nerve fibres and cells containing
> >>acetylcholinesterase on frozen sections of intestine.
> >>I found a method in an old book where the incubation takes between 7 and
> 30
> >>days! Is there anything simpler?
> >
> >Hi
> >
> >It's been a few years since I've done AChE histochemistry, but there are 
> >certainly many protocols a lot shorter than the one you mentionned. 
> >Personnally, I prefered the protocol published by Di Patre et al. in JHC 
> >41 (1993): 129-135. Very sensitive, easy and most reagents are cheap. I 
> >have a protocol, but unfortunately it's in French. Otherwise I can fax
> you 
> >the material and methods section of the reprint.
> >
> >Paul
> 
>  
> -=-
>                                                                         (o
> -) O
> =3D========================3D======oOo==(_)==OOo===========3D==================
> Paul Klosen, PhD
> CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
> Universite Louis Pasteur  12, rue de l'Universite
> F-67000 Strasbourg, FRANCE
> Tel. 03.88.35.85.04  Fax. 03.88.24.04.61
> =3D=======================klosen@neurochem.u-strasbg.fr================3D=====
> ====



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