We do the same (the Sigma Amylase Type VI-B) as a 0.6% solution in
distilled water for  10 mins at room temperature.

Barbara Albert
Umiversity of California
San Francisco Medical Center

At 22:42 05/13/2000 -0500, you wrote:
>Hi Eileen,
>	We use Sigma Amylase ( Type V1-B: from Porcine Pancreas ) We use it
>as a 1% solution in distilled water for 15mins at room temperature.
>	Alex Brown
>	Crosshouse Hospital
>	Kilmarnock, Scotland
>
> ----------
>From: Eileen_Dusek@cdh.org
>To: HistoNet@pathology.swmed.edu
>Subject: PAS DIASTASE
>Date: Thursday, May 11, 2000 5:21PM
>
>Hello everyone,
>Does anyone have a procedure for Pas Diastase without using saliva.  In
>this day and age of safety i am surprised we still use our own saliva.
>I know Sigma has an Amylase Type IX-A, is anyone familar with using this
>product, or any other type of diastase or amylase.
>Thank you all very much.
>
>Eileen  Dusek
>Central Dupage Hospital
>
>
>
>
>At 22:42 05/13/2000 -0500, Brown Alex wrote:
>Hi Eileen,
>	We use Sigma Amylase ( Type V1-B: from Porcine Pancreas ) We use it
>as a 1% solution in distilled water for 15mins at room temperature.
>	Alex Brown
>	Crosshouse Hospital
>	Kilmarnock, Scotland
>
> ----------
>From: Eileen_Dusek@cdh.org
>To: HistoNet@pathology.swmed.edu
>Subject: PAS DIASTASE
>Date: Thursday, May 11, 2000 5:21PM
>
>Hello everyone,
>Does anyone have a procedure for Pas Diastase without using saliva.  In
>this day and age of safety i am surprised we still use our own saliva.
>I know Sigma has an Amylase Type IX-A, is anyone familar with using this
>product, or any other type of diastase or amylase.
>Thank you all very much.
>
>Eileen  Dusek
>Central Dupage Hospital
>
>
>
>
>