I concur with Andrea: Ice works better than Histofreeze.  I usually protect
my fingers with a paper towel and hold the ice cube against the block face
in the (locked!) microtome.
                 Allen A. Smith
			Barry University
			 Miami Shores, FL		
-----Original Message-----
From: Horn, Hazel [mailto:HornHazelV@exchange.ach.uams.edu]
Sent: Tuesday, May 09, 2000 10:02 AM
To: 'AndreaH@imclone.com'; HistoNet Server
Subject: RE: Histofreeze


Andrea's right..........iced water is the best way for sectioning for me!
Hazel

> -----Original Message-----
> From:	AndreaH@imclone.com [SMTP:AndreaH@imclone.com]
> Sent:	Monday, May 08, 2000 08:49 AM
> To:	HistoNet Server
> Subject:	RE: Histofreeze
> 
> 
> I used to use a lot of Histofreeze spray and it became addictive to use
> but
> never really gave me better sections.  Then I discovered putting the
> blocks, after facing, on an iceblock that is melting a bit b/c it's at
> room
> temp and I have never used histofreeze sprays again ... better for me,
> better for the block, and I get beautiful sections every time even with
> finicky mouse tissues. My advice is to get rid of it. Plain ole ice works
> much better.
> 
> Andrea
>