Hi Marylou,
Just a quick note about the High Iron Diamine method.  We investigated a
number of years ago.  It is not hard to do, but the chemicals are EXTREMELY
toxic. Skin absorption and breathing in the dust when making solutions is a
problem too.  Look into this aspect before you proceed.  Gook Luck!
Best Regards.

Mickie

Michael L Johnson, BS, HTL(ASCP)
Histology Supervisor
Department of Pathology
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, WA 99220

johnsom@shmc.org


-----Original Message-----
From: Marylou Pohl [mailto:mbpohl@acsu.buffalo.edu]
Sent: Tuesday, May 09, 2000 8:38 PM
To: Histonet@pathology.swmed.edu
Subject: High iron diamine method


One of our pathologists went to a GI conference in Mass. last week.  She
came back with a request for a stain we have not done before:  High iron
diamine for sulfated mucins

I know it is in Sheehan's HISTOTECHNOLOGY (pg 175).

My question is:  Does anyone use this routinely?  That is the impression
I am getting from her conversation with me.

Thank you very much.