And i had a look in my lab book, and i did try using the antibody on
paraffins....and it was still pants.

emma c

> -----Original Message-----
> From:	Tracy Bartolotta [SMTP:tbartolo@med.unc.edu]
> Sent:	Tuesday, May 09, 2000 6:16 PM
> To:	histonet@pathology.swmed.edu
> Subject:	GFP antibodies?
> 
> I have an investigator who would like to try the Living Colors Peptide
> Antibody (cat.# 8367-1 from Clontech).  He wants to use it to detect
> Green Fluorescent Protein using DAB instead of it's fluorescence.  We
> have found a paper that got this antibody to work on 4% PFA fixed frozen
> tissue.  Does anyone know if it will also work on paraffin embedded
> tissue?  Or, if you have experience with another antibody to detect GFP
> I'd be interested.  I know this has been discussed before on histonet,
> but I can't seem to access the archives with the url address that I
> have.  Thank you.
> 
> Tracy Bartolotta
> UNC Cystic Fibrosis Center