RE: Fibroblasts.

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From:Simon Smith <>
To:"Histonet (E-mail)" <>
Content-Type:text/plain; charset="iso-8859-1"

Dako used to have an antibody to the beta subunit of proline-4-hydroxylase
called Fib 5B5.  Works well only on frozens or sainte-marie processed
tissue, but will also demonstrate other cells producing protein with lots of
disulphide bridges (plasma cells are culprits and I would guess hair
follicles would do it to).  You could always try some "antigen retrieval" if
you want it to do paraffins.   If you can find some commercially available,
try the alpha subunit of proline 4 hydroxylase, this is much more specific.
(Proline 4 hydroxylase is the enzyme that hydroxylates proline in collagen,
this helps increase the twist on the triple helix and keeps the collagen
from unravelling, as it does in scurvy).  As a result of this you will get a
lot of labelling in actively synthesizing fibroblasts, much less in
quiescent ones.



Simon Smith B.Sc. AIBMS
Supervisor, Laboratory Resources
Skeletech, Inc.
22002 26th Ave SE, Suite 104
Bothell   WA   98021
Voice: (425) 424 2663   Fax:  (425) 424 2600

-----Original Message-----
From: Jeffrey S Crews []
Sent: Saturday, May 06, 2000 1:40 PM
Subject: Re: Fibroblasts.

I believe we use anti-human vimentin. Not sure of the brand or protocol,
but I can find out if you want.

Jeffrey Crews, HTL (ASCP)
Organogenesis, Inc.

On Fri, 05 May 2000 09:36:52 +0100 "Dr. Ian Montgomery."
<> writes:
>	Anyone point me towards a good marker for fibroblasts.
>Dr. Ian Montgomery,
>West Medical Building,
>University of Glasgow,
>G12 8QQ.
>Tel: 0141 339 8855.  Extn:6602.
>Fax: 0141 330 2923

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