We always used the 1.5 thickness and my boss was always taking
photomicrographs.  He would not allow anything else to be used.  Pam Marcum

-----Original Message-----
From: Jennings, Margaret A. [mailto:jennings@mayo.edu]
Sent: Thursday, May 11, 2000 8:30 AM
To: histonet@pathology.swmed.edu
Subject: Coverglass thickness



I have to agree with Andi. coverglass thickness makes a big difference at
the research level. We have to take photos that are publishable quality.
Index of refraction etc. makes a big difference. Photos are not as forgiving
as your eyes.  I believe everything in the light path should be taken into
consideration including sample, coverglass, mountant and oil/air. The optics
in most scopes (please someone correct me if I am mistaken) are set for 1.5
standard coverglass especially for confocal microscopy. AJ











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