We had been using Dako's CD5 and just switched to Cell Marque's prediluted.
It works wonderfully and is very clean.  We use a steamer for HIER and
Dako's High pH target retrieval solution for 20 min and then 20 min cool
down.  The incubation is 30 min.  As I said, we have had beautiful results
on the Dako autostainer.

> ----------
> From: 	Marylou Pohl[SMTP:mbpohl@acsu.buffalo.edu]
> Sent: 	Sunday, April 30, 2000 3:19 PM
> To: 	Amos Brooks
> Cc: 	histonet
> Subject: 	Re: CD10 ???
> 
> <<File: mbpohl.vcf>>
> If you don't mind me piggy-backing on this message, along with CD10, CD5
> is
> also a hematopathology stain to contend with.  Same questions apply.
> 
> Marylou Pohl
> 
> Amos Brooks wrote:
> 
> > Hey All,
> >     One of our hematopathologists asked for an an antibody to be worked
> > up that we dont have yet. Does anyone have any suggestions for CD10 on
> > formalin fixed paraffin embedded sections. Please include which
> > detection kit you use and any pretreatments and titrations you use.
> > Thanks much,
> > Amos Brooks
>