I do use fresh reagents with each stain but I think it may be the
Richard-Allan mounting medium.  Jennifer MacDonald pointed out that it is
incompatible with picric acid!

-----Original Message-----
From: Mary Bryhan [mailto:mbryhan@northernhealth.org]
Sent: Tuesday, May 02, 2000 11:26 AM
To: jqb7@cdc.gov
Subject: Re: B&B Gram problem


I haven't seen that one, but what about your acetone before and after picric
acid/acetone - do you use fresh solution every time?  Additionally the
acetone/xylene before xylene... is that fresh.  It sounds like you aren't
dehydrating well enough, but I could be wrong.  Good luck!     

Mary Bryhan - Team Leader - Pathology
Northern Michigan Hospital
Petoskey,  Michigan