Are you using Richard Allen mounting media?  In the info it states that the
media is not compatible with picric acid stains?  Have you changed your
mounting media?

Jennifer MacDonald

> ----------
> From: 	Bartlett, Jeanine[SMTP:jqb7@cdc.gov]
> Sent: 	Tuesday, May 02, 2000 5:59 AM
> To: 	Histonet
> Subject: 	B&B Gram problem
> 
> Histonetters:
> 
> I have developed a problem that I am hoping someone out there in
> "Histoland"
> can help me with.  I have been using Newcomer and Polyscientific 0.1%
> aqueous picric acid/acetone for my Gram procedure for years.  In recent
> weeks a problem has developed.  After completing the picric acid/acetone
> step I have a nice yellowish background.  However, within minutes to hours
> if you go back and look at the coverslipped slide it has turned very pink
> and the there is a yellowish halo or blob that has shifted down the slide
> away from the tissue.  When you look under the scope there is no yellow in
> the tissue section any longer.  I tried a different bottle of picric
> acid/acetone and additional xylene at the end but so far it has not
> helped.
> 
> Has anyone else experienced this problem?
> 
> Thanks in advance for your help.
> 
> Jeanine Bartlett
> Centers for Disease Control
> Atlanta, GA
> jbartlett@cdc.gov 
> 
> 
>