Patsy,
We do IHC on frozen sections of lymph node for immunophenotyping of
lymphomas all the time. We use 0.3% hydrogen peroxide for 5 minutes, after
the sections have been fixed and hydrated.  If you put them in dry, they
will foam up and cause much artefact.  Hope this helps.
Mickie

Michael L Johnson, BS, HTL(ASCP)
Histology Supervisor
Department of Pathology
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, WA 99220

johnsom@shmc.org


-----Original Message-----
From: Patsy.Ruegg@UCHSC.edu [mailto:Patsy.Ruegg@UCHSC.edu]
Sent: Saturday, April 29, 2000 9:35 AM
To: histonet@pathology.swmed.edu
Subject: 2 ihc questions


1.  does anybody do pap detection on frozen sections, if you do how do you
block for endogenous peroxidase, in my previous life we always avoided
hydrogen peroxide on frozen sections because it chewed up the tissue so
badly??????  enlighten me.

2.  does anyone have experience with doing ihc on alcohol fixed tissue
samples?  we have tons of archived bone samples fixed in cold 100% methyl
alcohol to preserve the fluorchrome label, that we would like to do some IHC
on.  my past experience with this alcohol fixed tissue has been a dismal
failure.

thank you for your advice
patsy ruegg