Protein extraction from paraffin blocks
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From: | Victoria Baker <vbaker60@yahoo.com> |
To: | HistoNet Server <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi All!
The foundation is looking into extracting proteins
from paraffin/formalin fixed tissue samples. Thus
far, the success is eh to not so hot. I was wondering
if anyone had a procedure to do this, currently I
haven't found anything that really gave me an answer.
Given that the protein is masked by fixation, it seems
logical that their has to be a method to unmask it.
In IHC it's digestion, retrieval or both in a
combination. If anyone has a source I could tap into,
I'd really appreciate it.
Thanks.
Vikki Baker
American Health Foundation
Valhalla, New York
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