Protein extraction from paraffin blocks

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From:Victoria Baker <>
To:HistoNet Server <>
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Hi All!

The foundation is looking into extracting proteins
from paraffin/formalin fixed tissue samples.  Thus
far, the success is eh to not so hot.  I was wondering
if anyone had a procedure to do this, currently I
haven't found anything that really gave me an answer. 
Given that the protein is masked by fixation, it seems
logical that their has to be a method to unmask it. 
In IHC it's digestion, retrieval or both in a
combination.  If anyone has a source I could tap into,
I'd really appreciate it.


Vikki Baker
American Health Foundation
Valhalla, New York

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