Mayer's hematoxylin and chloral hydrate
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From: | Phyllis Davie <pdavie@phenopath.com> |
To: | "Histonet" <HistoNet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
I have been having trouble with the hematoxylin counterstain for my
immunos. It is generally weak, with poor nuclear detail. I have been
purchasing Mayer's hematoxylin, and I have tried 4 different vendors now,
all with the same results. My first question is; what can be causing
this weak staining, and what can I do to fix it? (I have been
counterstaining from 4-15 minutes, and many times, the staining--poor as
it is--drops off markedly after just a few racks of slides)
The second part of my question is this; We decided to make our own
Mayer's hematoxylin, but the recipe we found in the AFIP Laboratory
Methods in Histotechnology, p. 52, calls for 50g of chloral hydrate.
Unfortunately, chloral hydrate is a class IV DEA (Drug Enforcement
Agency) regulated substance, and no-one here has a DEA license. Can
anyone tell me what is the purpose of the chloral hydrate in the
hematoxylin is?
Thank you
Phyllis Davie
Clinical Laboratory Supervisor
PhenoPath Laboratories
Seattle, WA
pdavie@phenopath.com
(206)374-9009 phone
(206)374-9009 fax
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