Isolating dendrites in the hippocampus

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From:WPDyckman@lbl.gov
To:histonet@pathology.swmed.edu
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Hello all -

I would like to know how to best go about isolating and examining 
dendrite spines for atrophy (what stain is most appropriate, etc.)?  We 
are cutting sections of perfused mouse brains, and are interested in 
shifting our focus from brain region volumes to the condition of the 
dendrites in the hippocampal region.

Thanks,

Will




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